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Multispectral Microscopy

When employing optical characterization of human tissue it is important to obtain proper characteristics of all its constituents that coexist in a complex fashion. This requires that each constituent is isolated and measured independently to determine how it reacts to different wavelengths of light (primarily absorption). Besides the hyperspectral camera, we have a multispectral microscope which employs a range of LEDs in a broad spectral range (350 nm – 940 nm) that can independently illuminate a sample from which absorption and scattering characteristics can be determined.  Here we can obtain an even higher spatial resolution than the hyperspectral camera and can therefore potentially provide information about the absorbing chromophores that may exist in for example various cancer tissue.

Multispectral microscopy setup. Illustration.
Schematic cross section of an LED based multispectral microscope (right) showing three different excitation geometries that can generate images yielding different information (left). The three images to the left represent a single layer of red blood cells where some cells are infected with malaria.