Gustav Smith
Associate professor
Novel microRNA regulators of atrial natriuretic peptide production
Author
Summary, in English
Atrial natriuretic peptide (ANP) has a central role in regulating blood pressure in humans. Recently, microRNA 425 (miR-425) was found to regulate ANP production by binding to the mRNA of NPPA, the gene encoding ANP. mRNAs typically contain multiple predicted microRNA (miRNA)-binding sites, and binding of different miRNAs may independently or coordinately regulate the expression of any given mRNA. We used a multifaceted screening strategy that integrates bioinformatics, next-generation sequencing data, human genetic association data, and cellular models to identify additional functional NPPA-targeting miRNAs. Two novel miRNAs, miR-155 and miR-105, were found to modulate ANP production in human cardiomyocytes and target genetic variants whose minor alleles are associated with higher human plasma ANP levels. Both miR-155 and miR-105 repressed NPPA mRNA in an allele-specific manner, with the minor allele of each respective variant conferring resistance to the miRNA either by disruption of miRNA base pairing or by creation of wobble base pairing. Moreover, miR-155 enhanced the repressive effects of miR-425 on ANP production in human cardiomyocytes. Our study combines computational, genomic, and cellular tools to identify novel miRNA regulators of ANP production that could be targeted to raise ANP levels, which may have applications for the treatment of hypertension or heart failure.
Department/s
- Molecular Epidemiology and Cardiology
- Cardiovascular Research - Hypertension
- EXODIAB: Excellence of Diabetes Research in Sweden
- EpiHealth: Epidemiology for Health
Publishing year
2016
Language
English
Pages
1977-1987
Publication/Series
Molecular and Cellular Biology
Volume
36
Issue
14
Document type
Journal article
Publisher
American Society for Microbiology
Topic
- Cell and Molecular Biology
- Cardiac and Cardiovascular Systems
Status
Published
Research group
- Molecular Epidemiology and Cardiology
- Cardiovascular Research - Hypertension
ISBN/ISSN/Other
- ISSN: 0270-7306