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Professor Malin Malmsjö, MD, PhD. Photo.

Malin Malmsjö

Professor

Professor Malin Malmsjö, MD, PhD. Photo.

Quantitative Proteomics Indicate Radical Removal of Non-Small Cell Lung Cancer and Predict Outcome

Author

  • Embla Bodén
  • Jesper Andreasson
  • Gabriel Hirdman
  • Malin Malmsjö
  • Sandra Lindstedt

Summary, in English

Non-small cell lung cancer (NSCLC) is associated with low survival rates, often due to late diagnosis and lack of personalized medicine. Diagnosing and monitoring NSCLC using blood samples has lately gained interest due to its less invasive nature. In the present study, plasma was collected at three timepoints and analyzed using proximity extension assay technology and quantitative real-time polymerase chain reaction in patients with primary NSCLC stages IA–IIIA undergoing surgery. Results were adjusted for patient demographics, tumor, node, metastasis (TNM) stage, and multiple testing. Major histocompatibility (MHC) class 1 polypeptide-related sequence A/B (MIC-A/B) and tumor necrosis factor ligand superfamily member 6 (FASLG) were significantly increased post-surgery, suggesting radical removal of cancerous cells. Levels of hepatocyte growth factor (HGF) initially increased postoperatively but were later lowered, potentially indicating radical removal of malignant cells. The levels of FASLG in patients who later died or had a relapse of NSCLC were lower at all three timepoints compared to surviving patients without relapse, indicating that FASLG may be used as a prognostic biomarker. The biomarkers were confirmed using microarray data. In conclusion, quantitative proteomics could be used for NSCLC identification but may also provide information on radical surgical removal of NSCLC and post-surgical prognosis.

Department/s

  • Thoracic Surgery
  • WCMM-Wallenberg Centre for Molecular Medicine
  • Stem Cell Center
  • StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
  • DCD transplantation of lungs
  • LTH Profile Area: Photon Science and Technology
  • Department of Clinical Sciences, Lund
  • Clinical and experimental lung transplantation
  • LUCC: Lund University Cancer Centre
  • NPWT technology
  • Lung Bioengineering and Regeneration

Publishing year

2022-11

Language

English

Publication/Series

Biomedicines

Volume

10

Issue

11

Document type

Journal article

Publisher

MDPI AG

Topic

  • Cancer and Oncology
  • Clinical Laboratory Medicine

Keywords

  • biomarkers
  • non-small cell lung cancer
  • proteomics

Status

Published

Research group

  • DCD transplantation of lungs
  • Clinical and experimental lung transplantation
  • NPWT technology
  • Lung Bioengineering and Regeneration

ISBN/ISSN/Other

  • ISSN: 2227-9059