Magnus Cinthio
Senior lecturer
Photoacoustic Imaging of Enucleated Eyes from Patients with Uveal Melanoma can Reveal Extrascleral Growth
Author
Summary, in English
Background:
Uveal melanoma is treated by either enucleation (removal of the eye) or local eye-sparing therapies, depending on tumor size and whether there are signs of extrascleral growth. Photoacoustic (PA) imaging is a novel imaging modality that provides high-resolution images of the molecular composition of tissues.
Objective:
In this study, the feasibility of PA imaging for uveal melanomas and detection of extrascleral growth was explored.
Methods:
Seven enucleated human eyes with uveal melanomas were examined using PA imaging. The spectral signatures of the melanomas and the layers of the normal eyewall were characterized using 59 excitation wavelengths from 680 to 970 nm.
Results:
Significant differences were seen between the spectra obtained from melanoma and the healthy eyewall. Using spectral unmixing, melanin, hemoglobin and collagen could be mapped out, showing the architecture of the tumor in relation to the eyewall. This allowed visualization of regions where the tumor extended into the extrascleral space.
Conclusion:
PA imaging appears to have the potential to aid in assessing uveal melanomas and as a diagnostic tool for the detection of extrascleral growth.
Uveal melanoma is treated by either enucleation (removal of the eye) or local eye-sparing therapies, depending on tumor size and whether there are signs of extrascleral growth. Photoacoustic (PA) imaging is a novel imaging modality that provides high-resolution images of the molecular composition of tissues.
Objective:
In this study, the feasibility of PA imaging for uveal melanomas and detection of extrascleral growth was explored.
Methods:
Seven enucleated human eyes with uveal melanomas were examined using PA imaging. The spectral signatures of the melanomas and the layers of the normal eyewall were characterized using 59 excitation wavelengths from 680 to 970 nm.
Results:
Significant differences were seen between the spectra obtained from melanoma and the healthy eyewall. Using spectral unmixing, melanin, hemoglobin and collagen could be mapped out, showing the architecture of the tumor in relation to the eyewall. This allowed visualization of regions where the tumor extended into the extrascleral space.
Conclusion:
PA imaging appears to have the potential to aid in assessing uveal melanomas and as a diagnostic tool for the detection of extrascleral growth.
Department/s
- Ophthalmology Imaging Research Group
- Department of Biomedical Engineering
- NPWT technology
Publishing year
2021-12-23
Language
English
Pages
270-276
Publication/Series
Open Ophthalmology Journal
Volume
15
Issue
1
Document type
Journal article
Publisher
Bentham Science Publishers
Topic
- Ophthalmology
Status
Published
Research group
- Ophthalmology Imaging Research Group
- NPWT technology
ISBN/ISSN/Other
- ISSN: 1874-3641