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Bodil Gesslein, MSc, PhD. Photo.

Bodil Gesslein

Visiting research fellow

Bodil Gesslein, MSc, PhD. Photo.

Photoacoustic imaging of periorbital skin cancer ex vivo: unique spectral signatures of malignant melanoma, basal, and squamous cell carcinoma : Biomedical Optics Express

Author

  • Magne Tordengren Stridh
  • Jenny Hult
  • Aboma Merdasa
  • John Albinsson
  • Agnes Pekar-Lukacs
  • Bodil Gesslein
  • Ulf Dahlstrand
  • Karl Engelsberg
  • Johanna Berggren
  • Magnus Cinthio
  • Rafi Sheikh
  • Malin Malmsjö
Show all

Summary, in English

Radical excision of periorbital skin tumors is difficult without sacrificing excessive healthy tissue. Photoacoustic (PA) imaging is an emerging non-invasive biomedical imagi­­ng modality that has potential for intraoperative micrographic control of surgical margins. This is the first study to assess the feasibility of PA imaging for the detection of periocular skin cancer. Eleven patients underwent surgical excision of periocular skin cancer, one of which was a malignant melanoma (MM), eight were basal cell carcinomas (BCCs), and two squamous cell carcinomas (SCCs). Six tumors were located in the eyelid, and five in periocular skin. The excised samples, as well as healthy eyelid samples, were scanned with PA imaging postoperatively, using 59 wavelengths in the range 680–970 nm, to generate 3D multispectral images. Spectral unmixing was performed using endmember spectra for oxygenated and deoxygenated Hb, melanin, and collagen, to iden­­tify the chromophore composition of tumors and healthy eyelid tissue. After PA scanning, the tumor samples were examined histopathologically using standard hematoxylin and eosin staining. The PA spectra of healthy eyelid tissue were dominated by melanin in the skin, oxygenated and deoxygenated hemoglobin in the orbicularis oculi muscle, and collagen in the tarsal plate. Multiwavelength 3D scanning provided spectral information on the three tumor types. The spectrum from the MM was primarily reconstructed by the endmember melanin, while the SCCs showed contributions primarily from melanin, but also HbR and collagen. BCCs showed contributions from all four endmembers with a predominance of HbO2 and HbR. PA imaging may be used to distinguish different kinds of periocular skin tumors, paving the way for future intraoperative micrographic control.

Department/s

  • Ophthalmology Imaging Research Group
  • Department of Biomedical Engineering
  • Clinical and experimental lung transplantation
  • NPWT technology

Publishing year

2022

Language

English

Pages

410-425

Publication/Series

Biomed. Opt. Express

Volume

13

Issue

1

Document type

Journal article

Publisher

Optical Society of America

Topic

  • Ophthalmology
  • Dermatology and Venereal Diseases

Keywords

  • Diffuse reflectance spectroscopy
  • Imaging techniques
  • Medical imaging
  • Optical imaging
  • Photoacoustic imaging
  • Three dimensional imaging

Status

Published

Research group

  • Ophthalmology Imaging Research Group
  • Clinical and experimental lung transplantation
  • NPWT technology

ISBN/ISSN/Other

  • ISSN: 2156-7085