Diffuse fluorescence tomography for photodynamic therapy of tumors

Summary, in English

We develop and validate diffuse optical tomography methods based on interstitial fluorescence measurements for the reconstruction of fluorescent photosensitizing drug spatial distribution. We propose another phase in the tomographic reconstruction algorithm resulting in quantitatively more accurate reconstruction. This quantitative information can be used to monitor the temporal evolution of the photosensitizing drug concentration in tumor tissue with a final goal to predict the photodynamic treatment outcome and adjust the light dose in real time during the treatment. Capabilities and limitations of the developed reconstruction methods are analyzed in numerical simulations. Experimental validation is conducted on prostate tissue-mimicking phantoms with inclusions containing photosensitizer verteporfin, using a laser light delivery and collection system designed for clinical use.

• Author: Stefan Šušnjar; Daniyal Ghauri; Björn Thomasson; Sanathana Konugolu Venkata Sekar; Stefan Andersson-Engels; Johannes Swartling; Nina Reistad
• Department/s: LU Profile Area: Light and Materials; LTH Profile Area: Engineering Health; Atomic Physics; LUCC: Lund University Cancer Centre; LTH Profile Area: Photon Science and Technology
• Publishing year: 2025-03-19
• Language: English
• Publication/Series: SPIE Photonics West 2025 : Proceedings Volume 13320, Biomedical Light Scattering XV
• Volume: 13320
• Full text: - 3 MB; Download statistics
• Links: http://dx.doi.org/10.1117/12.3041146
• Document type: Conference paper
• Publisher: SPIE
• Topic: Atom and Molecular Physics and Optics
• Keywords: diffuse optical tomography,; fluorescence; photodynamic therapy; hybrid phantoms; reconstruction; photosensitizing drug; inverse problem
• Conference name: SPIE Photonics West 2025
• Conference date: 2025-01-25 - 2025-03-30
• Conference place: San Fransisco, United States
• Status: Published